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Figure 7. Reduced <t>Foxp1</t> levels in NOD.B10 Idd9.3 B-cell progenitors and MZ B cells. BM and spleen cells from NOD and NOD.B10 Idd9.3 mice (7–8 weeks old) were stained with antibodies for surface expres- sion of indicated B-cell populations followed by intracellular stain- ing with a Foxp1-specific antibody. Histograms show staining with anti-Foxp1 (filled histograms) or secondary antibody (nonfilled his- togram) in (A) gated pro-B (B220+CD43+IgM−) and pre-B cells (B220+CD43−IgM−) and (B) MZ B cells (B220+IgM+CD21hiCD23−) and FO B cells (B220+IgM+CD21intCD23+) from NOD (black) and NOD.B10 Idd9.3 (gray) mice. Numbers in histograms represent geometric mean fluo- rescence intensity (MFI) of Foxp1. Representative data of three experi- ments are shown.
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Jackson Immuno fitc conjugated donkey anti mouse
Figure 7. Reduced <t>Foxp1</t> levels in NOD.B10 Idd9.3 B-cell progenitors and MZ B cells. BM and spleen cells from NOD and NOD.B10 Idd9.3 mice (7–8 weeks old) were stained with antibodies for surface expres- sion of indicated B-cell populations followed by intracellular stain- ing with a Foxp1-specific antibody. Histograms show staining with anti-Foxp1 (filled histograms) or secondary antibody (nonfilled his- togram) in (A) gated pro-B (B220+CD43+IgM−) and pre-B cells (B220+CD43−IgM−) and (B) MZ B cells (B220+IgM+CD21hiCD23−) and FO B cells (B220+IgM+CD21intCD23+) from NOD (black) and NOD.B10 Idd9.3 (gray) mice. Numbers in histograms represent geometric mean fluo- rescence intensity (MFI) of Foxp1. Representative data of three experi- ments are shown.
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Jackson Immuno alexafluor488 conjugated donkey anti rabbit igg f ab 2 fragment
Figure 7. Reduced <t>Foxp1</t> levels in NOD.B10 Idd9.3 B-cell progenitors and MZ B cells. BM and spleen cells from NOD and NOD.B10 Idd9.3 mice (7–8 weeks old) were stained with antibodies for surface expres- sion of indicated B-cell populations followed by intracellular stain- ing with a Foxp1-specific antibody. Histograms show staining with anti-Foxp1 (filled histograms) or secondary antibody (nonfilled his- togram) in (A) gated pro-B (B220+CD43+IgM−) and pre-B cells (B220+CD43−IgM−) and (B) MZ B cells (B220+IgM+CD21hiCD23−) and FO B cells (B220+IgM+CD21intCD23+) from NOD (black) and NOD.B10 Idd9.3 (gray) mice. Numbers in histograms represent geometric mean fluo- rescence intensity (MFI) of Foxp1. Representative data of three experi- ments are shown.
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Cell Signaling Technology Inc rabbit monoclonal anti cnpase
Figure 7. Reduced <t>Foxp1</t> levels in NOD.B10 Idd9.3 B-cell progenitors and MZ B cells. BM and spleen cells from NOD and NOD.B10 Idd9.3 mice (7–8 weeks old) were stained with antibodies for surface expres- sion of indicated B-cell populations followed by intracellular stain- ing with a Foxp1-specific antibody. Histograms show staining with anti-Foxp1 (filled histograms) or secondary antibody (nonfilled his- togram) in (A) gated pro-B (B220+CD43+IgM−) and pre-B cells (B220+CD43−IgM−) and (B) MZ B cells (B220+IgM+CD21hiCD23−) and FO B cells (B220+IgM+CD21intCD23+) from NOD (black) and NOD.B10 Idd9.3 (gray) mice. Numbers in histograms represent geometric mean fluo- rescence intensity (MFI) of Foxp1. Representative data of three experi- ments are shown.
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Figure 7. Reduced Foxp1 levels in NOD.B10 Idd9.3 B-cell progenitors and MZ B cells. BM and spleen cells from NOD and NOD.B10 Idd9.3 mice (7–8 weeks old) were stained with antibodies for surface expres- sion of indicated B-cell populations followed by intracellular stain- ing with a Foxp1-specific antibody. Histograms show staining with anti-Foxp1 (filled histograms) or secondary antibody (nonfilled his- togram) in (A) gated pro-B (B220+CD43+IgM−) and pre-B cells (B220+CD43−IgM−) and (B) MZ B cells (B220+IgM+CD21hiCD23−) and FO B cells (B220+IgM+CD21intCD23+) from NOD (black) and NOD.B10 Idd9.3 (gray) mice. Numbers in histograms represent geometric mean fluo- rescence intensity (MFI) of Foxp1. Representative data of three experi- ments are shown.

Journal: European journal of immunology

Article Title: The type 1 diabetes resistance locus B10 Idd9.3 mediates impaired B-cell lymphopoiesis and implicates microRNA-34a in diabetes protection.

doi: 10.1002/eji.201344116

Figure Lengend Snippet: Figure 7. Reduced Foxp1 levels in NOD.B10 Idd9.3 B-cell progenitors and MZ B cells. BM and spleen cells from NOD and NOD.B10 Idd9.3 mice (7–8 weeks old) were stained with antibodies for surface expres- sion of indicated B-cell populations followed by intracellular stain- ing with a Foxp1-specific antibody. Histograms show staining with anti-Foxp1 (filled histograms) or secondary antibody (nonfilled his- togram) in (A) gated pro-B (B220+CD43+IgM−) and pre-B cells (B220+CD43−IgM−) and (B) MZ B cells (B220+IgM+CD21hiCD23−) and FO B cells (B220+IgM+CD21intCD23+) from NOD (black) and NOD.B10 Idd9.3 (gray) mice. Numbers in histograms represent geometric mean fluo- rescence intensity (MFI) of Foxp1. Representative data of three experi- ments are shown.

Article Snippet: Cells were subsequently stained with Foxp1 (1:200; #2005, Cell Signaling Technology), followed by IgG (H+L), F(ab′)2 fragments (1:1000; #4414, Cell Signaling Technology), 30 min each.

Techniques: Staining